RNA isolation using Trizol

RNA isolation using Trizol RNA isolation using Trizol.docx 본문 RNA isolation using Trizol (cell : in culture) 1.Using at least 106 cells, aspirate off the media and wash X1 with ice cold PBS (1-2 ml) 2.Aspirate off the PBS (Remove as much as possible) and add 1 ml of trizol 본문내용 wash X1 with ice cold PBS (1-2 ml) Aspirate off the PBS (Remove as much as possible) and add 1 ml of trizol Scrape the plate briefly, then remove the trizol with a pipette and deposit the trizol/cell lysate into a 1.5 ml eppendorf tube. Let sit for 5 min at room temperature. Add 250 μl of chloroform and shake the tube vigorously for about 15 seconds. Let sit for 5 min at room temp. Centrifμge at 하고 싶은 말 RNA 분리 실험방법입니다.