H&E staining

H&E staining H&E staining.docx 본문 H&E staining protocol (For cryosection, Mouse brain tissue) 1. Deparaffin (frozen section slide) 본문내용 lene(1)3min Xylene(2)3min Xylene(3)3min 2. Hydrate 100% ethanol1min. 90% ethanol1min. 70% ethanol1min. 40(50)% ethanol.1min. 3. Staining (Nucleic acid) Hematoxylin.10min. (brain->over 10min.) (현미경관찰 후, 발색도가 높으면 stop.) 4. Wash in tap water. (Do not overwash!) 5. 1%(or 0.5%)HCl/70%etha 하고 싶은 말 H&E염색법 실험방법입니다.